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Related Concept Videos

Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Related Experiment Video

Updated: Jul 11, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

Maize genome sequencing by methylation filtration.

Lance E Palmer1, Pablo D Rabinowicz, Andrew L O'Shaughnessy

  • 1Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

Science (New York, N.Y.)
|December 20, 2003
PubMed
Summary

Methylation filtration of maize sequences provides a more complete gene set than other methods. This approach aids in identifying active transposons and organelle genomes for transcriptome completion.

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Last Updated: Jul 11, 2026

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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
07:26

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Published on: July 29, 2019

Area of Science:

  • Plant genomics
  • Molecular biology

Background:

  • Sequencing large, repetitive genomes like maize is challenging.
  • Gene enrichment strategies offer a more efficient alternative.

Purpose of the Study:

  • To evaluate methylation filtration as a gene enrichment strategy for maize.
  • To compare its effectiveness against other sequencing methods.

Main Methods:

  • Generation and analysis of approximately 100,000 undermethylated maize sequences.
  • Comparative analysis with the rice genome.

Main Results:

  • Methylation filtration yielded a more comprehensive maize gene representation compared to expressed sequence tags or transposon insertion sites.
  • Approximately 7% of repetitive DNA was unmethylated and selected.
  • Potentially active transposons and unmethylated organelle genomes were identified.

Conclusions:

  • Methylation filtration is an effective gene enrichment strategy for maize.
  • This method facilitates the identification of specific DNA elements and aids in transcriptome completion using reverse transcription polymerase chain reaction.