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Updated: Aug 29, 2026
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Expression, purification, crystallization and preliminary crystallographic analysis of the diarrhoea-causing and
R Deepa1, M R Jagannath, M M Kesavulu
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Abstract:
The region spanning the tetrameric coiled-coil domain and the interspecies-variable virulence-determining region of the cytoplasmic tail of rotaviral nonstructural protein NSP4 has been crystallized. The crystals belong to space group I222, with unit-cell parameters a = 30.70, b = 38.07, c = 181.62 A, and contain two molecules in the asymmetric unit. Diffraction data have been collected utilizing a MAR imaging plate to a resolution of 2.2 A. The tetramer is generated by the crystallographic dyad along the c axis.
