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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
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[Acute diarrhea in children less than 3 years of age: Enteropathogens isolated in patient's stools, compared with a

M S Toporovski1, I M Mimica, P P Chieffi

  • 1Santa Casa de São Paulo, São Paulo, SP, Brazil.

Jornal De Pediatria
|December 20, 2003
PubMed

Insights

Rotavirus was the most frequent cause of acute diarrhea (AD) in young children, followed by Shigella sp. in cases with bloody stools. Certain E. coli strains were highly pathogenic, but only when identified with specific tests.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Microbiology

Background:

  • Acute diarrhea (AD) is a significant health concern in young children.
  • Identifying the etiological agents of AD is crucial for effective treatment and prevention.

Purpose of the Study:

  • To determine the prevalence of various enteropathogens in children with acute diarrhea.
  • To compare the occurrence of these agents in patients versus a control group.

Main Methods:

  • A case-control study involving 100 children with AD and 100 controls (under three years old).
  • Stool specimen analysis for Rotavirus, specific Escherichia coli strains (EPEC, ETEC, EIEC, EHEC), Salmonella, Shigella, Campylobacter, Yersinia, Cryptosporidium, Giardia, and Entamoeba.
  • Statistical analysis using the exact Fisher test (p<0.05).

Main Results:

  • Rotavirus was the most common agent (21% in AD group vs. 3% in controls, p=0.0001).
  • Shigella sp. was found in 7% of AD cases but none of the controls (p=0.0140).
  • Classical EPEC subgroups (O55, O111, O119) were exclusively detected in AD patients, unlike general EPEC detection.

Conclusions:

  • Rotavirus is the leading cause of acute diarrhea in this pediatric population.
  • Rotavirus and Shigella sp. demonstrate high pathogenicity for acute diarrhea.
  • Standard EPEC detection using polyvalent sera is insufficient; monovalent antisera are needed to identify pathogenic strains.

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