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Updated: Aug 13, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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Down-regulation of platelet-derived growth factor receptor signaling during myogenesis

T Fiaschi1, P Chiarugi, F Buricchi

  • 1Dipartimento di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy.

Cellular and Molecular Life Sciences : CMLS
|December 20, 2003
PubMed

Insights

Muscle cell differentiation involves decreased platelet-derived growth factor receptor (PDGFr) signaling, not by changing receptor levels, but by reducing receptor phosphorylation. This is linked to increased phosphotyrosine phosphatase (PTP) activity.

Area of Science:

  • Cell biology
  • Molecular signaling

Background:

  • Cell differentiation often halts cell cycle progression.
  • Growth factor signaling impairment in differentiated cells is known.
  • Mechanisms include growth factor withdrawal or receptor downregulation.

Purpose of the Study:

  • Investigate alternative pathways for growth factor signaling downregulation during differentiation.
  • Propose that decreased receptor activation inhibits cell division pathways.
  • Focus on platelet-derived growth factor receptor (PDGFr) signaling in muscle differentiation.

Main Methods:

  • Studied PDGFr signaling during muscle differentiation (myogenesis).
  • Assessed PDGFr expression levels and tyrosine phosphorylation.
  • Examined phosphotyrosine phosphatase (PTP) expression and activity.

Main Results:

  • PDGFr signaling decreased during myogenesis without changing receptor expression.
  • Inhibition occurred via reduced PDGFr tyrosine phosphorylation (Tyr716, Tyr751, Tyr857, Tyr1021).
  • Increased PTP expression and activation were observed during myogenesis.

Conclusions:

  • PDGFr signaling is downregulated during muscle differentiation through reduced receptor phosphorylation.
  • Increased PTP activity is causally linked to PDGFr dephosphorylation.
  • This provides an alternative mechanism for inhibiting cell division signaling during differentiation.

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