Voltammetric study of glibenclamide at carbon paste and Sephadex-modified carbon paste electrodes

The oxidative behaviour of the antidiabetic agent glibenclamide on a bare carbon paste electrode (CPE) and a Sephadex-modified carbon paste electrode (SMCPE) was explored by cyclic and differential pulse voltammetry (DPV). The analysis procedure consisted of an open circuit accumulation step in stirred sample solution of Britton-Robinson buffer (0.04 mol L(-1), pH 2.0). This was followed by medium exchange to a clean solution of Britton-Robinson buffer (0.04 mol L(-1), pH 5.0), and subsequently an anodic potential scan was effected to obtain the voltammetric peak. The glibenclamide oxidation peak current obtained by DPV was proportional to the concentration of the glibenclamide in the range of 1.0 x 10(-9) mol L(-1) to 5.0 x 10(-8) mol L(-1) for 180 s accumulation time, with a detection limit of 4.0 x 10(-10) mol L(-1). A method was developed for the determination of glibenclamide in formulation and spiked human serum. Moreover, the proposed procedure was used to estimate the serum concentrations after oral administration of a 5 mg tablet of glibenclamide to three diabetic subjects.