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Updated: Aug 29, 2026
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
Published on: August 4, 2013
Using a vortex mixer for testicular sperm collection
Osamu Tokuyama1, Yoshihiro Nakamura, Koji Ozaki
1Department of Obstetrics and Gynecology, Osaka City University Medical School, Towako Reproduction Center Osaka, Osaka, Japan. otoku@med.osaka-cu.ac.jp
Objective:
To evaluate 2 methods of processing testicular tissue for the retrieval of viable sperm from men with nonobstructive azoospermia.
Study Design:
Fresh testicular tissue was obtained from nonobstructive azoospermia patients using a biopsy needle. The specimens were divided into 2 fractions. All specimens were minced and immersed in human tubal fluid (HTF). The first fraction was filtered through a nylon filter and incubated for 3 hours. The supernatant was centrifuged, resuspended in HTF and analyzed. The second fraction was immediately vortexed for 5 minutes and filtered through a nylon filter. The supernatant was centrifuged, resuspended in HTF and analyzed.
Results:
Spermatozoa were obtained in 13 of 24 cases (54.2%) using the vortex method and in 5 of 24 cases (20.8%) with the nylon filter method.
Conclusion:
The vortex mixing method may be a better option than the conventional method for processing testicular tissue for sperm collection.
