Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Internalization at glutamatergic synapses during development.

Ronald S Petralia1, Ya-Xian Wang, Robert J Wenthold

  • 1Laboratory of Neuroscience, NIDCD/NIH, 50/4142, 50 South Drive MSC 8027, Bethesda, MD 20892-8027, USA. petralia@nidcd.nih.gov

The European Journal of Neuroscience
|December 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular mechanism establishing the OFF pathway in vision.

Nature communications·2025
Same author

SLC26A4-AP-2 mu2 interaction regulates SLC26A4 plasma membrane abundance in the endolymphatic sac.

Science advances·2024
Same author

An initial HOPS-mediated fusion event is critical for autophagosome transport initiation from the axon terminal.

Autophagy·2024
Same author

Alzheimer's disease/dementia-associated brain pathology in aging DPP6-KO mice.

Neurobiology of disease·2022
Same author

NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals.

iScience·2022
Same author

BAX regulates dendritic spine development via mitochondrial fusion.

Neuroscience research·2022

Clathrin-coated pits internalize glutamate receptors at and away from synapses. This study reveals these pits are located extrasynaptically, internalizing N-methyl-d-aspartate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Plasticity

Background:

  • Glutamate receptors mediate fast excitatory neurotransmission.
  • Receptor internalization via clathrin-coated pits is a key mechanism for regulating synaptic function.
  • The precise location of glutamate receptor internalization relative to the synapse remains unclear.

Purpose of the Study:

  • To investigate the subcellular localization of clathrin-coated pits involved in glutamate receptor internalization.
  • To determine if receptor internalization occurs at or away from the synaptic active zone.
  • To identify the specific glutamate receptors internalized at extrasynaptic sites.

Main Methods:

  • Immunogold localization in rat brain (hippocampus).
  • Electron microscopy to visualize clathrin-coated pits and receptor localization.

Related Experiment Videos

  • Distinguishing between synaptic and extrasynaptic sites.

    • Main Results:

    • Clathrin-coated pits were found at the synaptic active zone edge and at more distant postsynaptic locations.
    • These pits were specifically localized to glutamatergic synapses.
    • Internalization of N-methyl-d-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors was observed at extrasynaptic sites.
    • Caveolin presence at excitatory synapses was noted, but its role is undetermined.

    Conclusions:

    • Glutamate receptor internalization occurs both synaptically and extrasynaptically via clathrin-coated pits.
    • Extrasynaptic internalization sites can be distant from the synapse.
    • Caveolin may play a role in excitatory synapse function, warranting further investigation.