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[Studies on the changes in expression of hypoxia induction factor-1alpha in myocardial tissue in severely scalded
Yong-Ming Dang1, Yue-Sheng Huang, Li-Feng Chen
1Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
Objective:
To investigate the changes in the expression of hypoxia induction factor-1alpha (HIF1-alpha) in myocardial tissues in severely scalded rats during early postburn stage.
Methods:
Male Wistar rats inflicted with 40% TBSA III degree scald were employed as the model. The myocardial tissue samples were harvested from the left and right ventricles at different postburn time points, and samples were also obtained from normal rats as control. The mRNA and protein expressions of HIF-1alpha in rat myocardial tissue were determined by RT-PCR and Western blot analysis respectively.
Results:
There was a difference of HIF-1alpha expression between left and right ventricles of the normal rats at both transcriptional and translational levels, and the mRNA and protein expressions of HIF-1alpha in the myocardial tissue of scalded rats were increased dramatically at early postburn stage.
Conclusion:
The tolerance to ischemia and hypoxia of the rat left ventricle was higher than that of the right ventricle under normal condition. An increase in HIF1alpha expression in rat myocardial tissue could be induced in severely scalded rats.
