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Updated: Aug 29, 2026

Swine Model of Biofilm Infection and Invisible Wounds
Published on: June 16, 2023
[The clinical staging and tissue bacterial quantification in the diagnosis of burn wound sepsis]
Zhi-Qiang Wang1, Bao-Ren Cai, Jie Xiao
1Department of Burns and plastic surgery, The 205th Hospital of People's Liberation Army, Jinzhou 121001, Liao Ning Province, P.R. China.
Objective:
To investigate and re-evaluate the relationship between burn wound sepsis and tissue bacterial quantity.
Methods:
Thirty-two patients admitted during past 5 years were enrolled in the study. Bacterial isolation and quantity in burn wound tissue were carried out. Meanwhile clinical signs were evaluated for the staging of burn wound sepsis.
Results:
1) Bacterial invasion could be identified in 123 pieces of tissue samples from 32 patients. Samples with tissue bacterial quantity > or = 10(5)/g were found in 82 subeschar tissue samples, and 41 samples with bacteria <10(5)/g. Subeschar tissue samples with bacterial quantity > or = 10(5)/g could be determined in 68 samples from 18 patients, and < 10(5)/g in 20 samples from 5 cases. In addition, samples of subeschar tissue with bacterial quantity > or = 10(5)/g could only be found in some of the samples form 9 cases. 2) Burn wound sepsis could be classified into I-IV stages according to tissue bacterial quantification and clinical signs.
Conclusion:
Burn wound sepsis could be established by identification of bacterial invasion into living tissue with clinical symptoms of toxemia.

