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Updated: Aug 29, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
2,5-Hexanedione inhibits rat brain acetylcholinesterase activity in vitro
Maria E Pereira1, Andréa I H Adams, Nélson S Silva
1Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. pereirame@yahoo.com.br
Abstract:
A number of C(4)-C9 aliphatic ketones, including 2,5-hexanedione (2,5-HD), are acetylcholinesterase (AChE; EC 3.1.1.7) inhibitors. In the previous work, we demonstrated that antinociception induced by 2,5-HD was partially reversed by atropine. Since cholinergic system seems to be affected by hexacarbon compounds, in the present study we examined the effect of 2,5-HD on AChE activity of rat brain in vitro. The results demonstrated that both Km and Vmax were altered by 2,5-HD. At 5 mM 2,5-HD, there were no significant changes in Km or Vmax parameters, but at concentrations higher than 10 mM an increase in Km and a decrease in Vmax was observed. The Ki plot was linear and the curves intersect on the left of the 1/V versus [I] plot. These data characterize an inhibition of the mixed type. Although the 2,5-HD concentration that inhibited AChE activity is high compared to that attainable in occupationally exposed workers, these data are useful in understanding the neurotoxicity of this compound. Furthermore, the effect of 2,5-HD on AChE activity must not be a consequence of its effects as an organic solvent on protein structure, because total inhibition induced by 2,5-HD was partially reversed by increasing substrate concentration.
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