Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhancing and diminishing gene function in human embryonic stem cells.

Ludovic Vallier1, Peter J Rugg-Gunn, Isabelle A Bouhon

  • 1Department of Surgery, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK. lv225@cam.ac.uk

Stem Cells (Dayton, Ohio)
|December 23, 2003
PubMed
Summary

Researchers developed a lipofection method to create fluorescent human embryonic stem (hES) cell lines. This technique enables gene function studies using small interfering (si)RNAs for gene knockdown, aiding in understanding gene roles and cell differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poising and connectivity of emergent human developmental enhancers in the transition from naive to primed pluripotency.

Cell reports·2026
Same author

Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cells.

Nucleic acids research·2026
Same author

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Genome biology·2026
Same author

Unmasking metabolic disruptors: The NEMESIS project's quest for Novel Biomarkers, Evidence on Adverse Effects, and Efficient Methodologies.

Open research Europe·2026
Same author

Modeling human embryo implantation in vitro.

Cell·2025
Same author

Author Correction: Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025

Area of Science:

  • Stem Cell Biology
  • Molecular Genetics

Background:

  • Gain- and loss-of-function studies are crucial for gene function analysis.
  • Human embryonic stem (hES) cells are valuable models for developmental biology.

Purpose of the Study:

  • To establish efficient gain- and loss-of-function methods in hES cells.
  • To generate fluorescent hES cell lines for tracking and manipulation.
  • To validate small interfering (si)RNA for gene knockdown in hES cells.

Main Methods:

  • Lipofection was used to introduce fluorescent proteins into hES cells.
  • Generated stable hES cell lines expressing green or red fluorescent proteins.
  • Utilized small interfering (si)RNAs to achieve gene function knockdown.

Main Results:

Related Experiment Videos

  • Successfully created fluorescent hES cell lines without compromising pluripotency.
  • Demonstrated effective gene expression knockdown using siRNAs in hES cells.
  • Showed stable siRNA expression can reduce endogenous gene expression.

Conclusions:

  • The developed lipofection approach is efficient for creating modified hES cells.
  • These methods facilitate robust gene function studies in hES cells.
  • The techniques have broad applications in human gene discovery and differentiation modulation.