High efficiency gene transfer into mammalian kidney cells using baculovirus vectors

Gene delivery into kidney cells is essential to the development of gene therapy for nephropathy. This paper describes the use of baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV) as a vector for gene delivery into several mammalian kidney cells. High-level expression of a reporter gene encoding for the green fluorescent protein (GFP) under a heterogonous promoter was observed in kidney cells from mouse, hamster, monkey, pig, and human. The level of transgene expression exhibited viral dose dependence and was enhanced by the addition of butyrate. Baculovirus transduction could also provided long-term target gene expression in kidney cell lines without cytotoxic effects. High efficiency transduction was also observed in primary mouse kidney cells. These results indicate that baculovirus can be used as a vector for kidney-directed gene transfer.