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[Effects of daidzein on sperm quality, testis gain and testosterone in mice]
Zhao-Yu Han1, Gen-Lin Wang, Wen Chen
1College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Objective:
To evaluate the effects of daidzein on sperm quality of male mice.
Methods:
Three different doses of daidzein were supplemented to pubertal male mice for 21 days(5 mg/kg, 100 mg/kg, 1,000 mg/kg ration). The viability of sperm was determined by eosin-Y, the acrosome was observed by Wright-Giemsa staining, and the testosterone was measured by radioimmunoassay.
Results:
Daidzein at the dose of 5 mg/kg ration significantly increased serum testosterone levels (P < 0.01), prompted testis gain (P < 0.05), and improved spermatozoa quality. Daidzein at dose of 1,000 mg/kg ration could inhibit the secretion of serum testosterone (P < 0.01), without significant variation in spermatozoa quality. Daidzein at dose of 100 mg/kg ration did not significantly affect sperm quality and other index.
Conclusions:
Daidzein can affect sperm quality and in dosage-dependant ways.
