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Updated: Aug 29, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
[Study on DNA methylation status of WT1 gene promoter in leukemia cell]
Quan-shun Wang1, Li Yu, Yu Zhao
1Department of Hematology, PLA General Hospital, Beijing 100853, China.
Objective:
To analyse the WT1 expression and its DNA methylation status of its promoter domain.
Method:
The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
Results:
WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.
Conclusion:
DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.
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