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Related Experiment Videos

Non-primate lentiviral vector administration in the TMJ.

S Kyrkanides1, P Kambylafkas, J H Miller

  • 1Eastman Department of Dentistry, University of Rochester, 625 Elmwood Ave., Rochester, NY 14620, USA. stephanos_kyrkanides@urmc.rochester.edu

Journal of Dental Research
|December 24, 2003
PubMed
Summary

Gene therapy shows promise for temporomandibular joint (TMJ) disorders. Lentiviral vectors successfully targeted TMJ tissues and sensory neurons, suggesting potential for pain management gene transfer.

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Area of Science:

  • Biomedical Engineering
  • Gene Therapy
  • Otolaryngology

Background:

  • Temporomandibular joint (TMJ) disorders represent a significant clinical challenge.
  • Gene therapy offers a novel therapeutic avenue for managing TMJ disorders.
  • Understanding the efficacy of gene delivery vectors within the TMJ is crucial.

Purpose of the Study:

  • To evaluate the effects and efficacy of lentiviral vectors in the temporomandibular joint.
  • To determine the biodistribution and transduction capabilities of lentiviral vectors within TMJ tissues.
  • To explore the potential of lentiviral vectors for targeted gene delivery in the TMJ.

Main Methods:

  • Injection of a defective feline immunodeficiency virus (FIV) vector carrying the lacZ reporter gene into the articular joint space.

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  • Analysis of lacZ expression using Polymerase Chain Reaction (PCR), X-gal histochemistry, and beta-galactosidase immunocytochemistry.
  • Assessment of vector transduction in hard and soft tissues of the TMJ and associated neural pathways.

    • Main Results:

    • Successful transduction of both hard and soft tissues within the temporomandibular joint.
    • Detection of reporter gene expression in a subset of primary sensory neurons of the ipsilateral trigeminal ganglion.
    • Evidence of retrograde transport of the lentiviral vector from peripheral nerve fibers to the neuronal nucleus.

    Conclusions:

    • Lentiviral vectors demonstrate efficient gene transfer capabilities within the temporomandibular joint.
    • The observed transduction of trigeminal ganglion neurons suggests potential for targeting pain pathways.
    • Lentiviral vectors represent a promising platform for gene therapy applications in managing TMJ pain.