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Related Experiment Videos

Extended histopathology in immunotoxicity testing: interlaboratory validation studies.

D R Germolec1, A Nyska, M Kashon

  • 1Laboratory of Molecular Toxicology/National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. germolec@niehs.nih.gov

Toxicological Sciences : an Official Journal of the Society of Toxicology
|December 24, 2003
PubMed
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Expanded histopathology shows promise for immunotoxicity screening. Pathologist agreement varied by tissue and lesion, highlighting the need for standardized training in assessing lymphoid tissue changes.

Area of Science:

  • Toxicology
  • Immunology
  • Pathology

Background:

  • Expanded histopathology is of interest for immunotoxicity assessment.
  • Semiquantitative histopathology can identify structural and architectural changes in lymphoid tissues.

Purpose of the Study:

  • To validate the utility of a semiquantitative histopathology approach for immunotoxicity assessment.
  • To evaluate interlaboratory reproducibility of extended histopathology for immune tissues.

Main Methods:

  • Utilized tissues from ten test chemicals and controls from the National Toxicology Program's immunotoxicology testing program.
  • Examined consistency among experienced toxicologic pathologists with varied expertise.
  • Assessed sensitivity of histopathological endpoints to detect chemical effects and dose response using factor analysis and ANOVA.

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Main Results:

  • Pathologist agreement was highest for thymus cortical cellularity, followed by spleen follicular cellularity and germinal center development in spleen and lymph nodes.
  • Agreement was poorest for spleen red-pulp changes.
  • Histopathological assessment ability depended on pathologist experience and lesion severity.

Conclusions:

  • Expanded histopathology is a potentially useful tool for immunotoxicity screening.
  • Interlaboratory reproducibility varies, influenced by pathologist expertise and specific lymphoid tissue lesions.
  • Standardized training is crucial for consistent immunotoxicity assessment using histopathology.