Related Experiment Video

Updated: Aug 29, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

In vitro proteoglycan sulfation derived from sulfhydryl compounds in sulfate transporter chondrodysplasias

Antonio Rossi1, Giuseppe Cetta, Rocco Piazza

  • 1Dipartimento di Biochimica Alessandro Castellani, Università di Pavia, Pavia, Italy. antrossi@unipv.it

Pediatric Pathology & Molecular Medicine
|December 25, 2003
PubMed

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Proteoglycans

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Glycosaminoglycans

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Hyaluronic...
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Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
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Protein Glycosylation

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Protein Modifications in the RER

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