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Published on: February 15, 2019
[Study on the inhibition of alpha-glucosidase by soyasaponins]
Jishu Quan1, Xuezhe Yin, Ming Jin
1Yanbian University, Yanji 133000.
Objective:
To study the inhibitory effects of soyasaponins on alpha-glucosidase (EC3.2.1.20).
Methods:
Soyasaponins were isolated by ODS column chromatography and high-performance liquid chromatography (HPLC) from hypocotyls of soybean. The inhibitory activities of each component of soyasaponins against alpha-glucosidase were tested by colorimetric method.
Results:
Soyasaponins showed potent inhibitory activities against alpha-glucosidase. Group B, group E and DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins showed stronger potency, which were non-competitive inhibitors of alpha-glucosidase with IC50 values of 10-40 mumol/L. While group A saponins showed a little lower potency with IC50 values of about 2 mmol/L.
Conclusion:
The results suggest soyasaponins, which exhibit inhibitory effects on alpha-glucosidase, seem physiologically useful for suppressing postprandial hyperglycemia in patients with diabetes mellitus.
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