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Related Experiment Videos

CD177 polymorphisms: correlation between high-frequency single nucleotide polymorphisms and neutrophil surface

Lorraine Caruccio1, Kelly Walkovich, Maria Bettinotti

  • 1Department of Transfusion Medicine, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.

Transfusion
|December 25, 2003
PubMed
Summary

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The human neutrophil antigen-2a (NB1) and PRV-1 are alleles of the CD177 gene. A common single nucleotide polymorphism (SNP) in CD177 may affect NB1 glycoprotein expression on neutrophils.

Area of Science:

  • Immunogenetics
  • Hematology

Background:

  • Human neutrophil antigen-2a (NB1) is a glycoprotein expressed on neutrophils.
  • PRV-1 is over-expressed in polycythemia rubra vera neutrophils.
  • NB1 and PRV-1 genes differ by four nucleotides.

Purpose of the Study:

  • Determine if PRV-1 and NB1 are alleles of the same gene or separate genes.
  • Investigate potential correlations between CD177 alleles and NB1 glycoprotein expression.

Main Methods:

  • Amplification and sequencing of DNA regions surrounding NB1 polymorphic sites.
  • Flow cytometry to assess CD177 bright-staining neutrophil populations.

Main Results:

  • PRV-1 and NB1 are alleles of the CD177 gene, as evidenced by homozygous PRV-1 polymorphisms in donors.

Related Experiment Videos

  • 13 single nucleotide polymorphisms (SNPs) resulting in amino acid changes were identified.
  • The CD177 G42C polymorphism was most common, with 42C/C donors showing larger CD177 bright-staining neutrophil populations.

    • Conclusions:

    • PRV-1 and NB1 are alleles of the polymorphic CD177 gene.
    • The G42C SNP in CD177 may influence NB1 glycoprotein expression on neutrophils.