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Related Experiment Videos

Antioxidative vitamins decrease cytotoxicity of HEMA and TEGDMA in cultured cell lines.

U I Walther1, I I Siagian, S C Walther

  • 1Walther-Straub-Institut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universität, Nussbaumstr. 26, 80336 München, Germany. udo.walther@lrz.uni-muenchen.de

Archives of Oral Biology
|December 25, 2003
PubMed
Summary
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Antioxidative vitamins, including Vitamin C and E, effectively reduced the toxicity of 2-hydroxyethylmethacrylate (HEMA) and triethleneglycoldimethacrylate (TEGDMA) in cell models. Glutathione depletion proved more sensitive than protein synthesis inhibition for assessing this toxicity.

Area of Science:

  • Biomaterials Science
  • Toxicology
  • Cell Biology

Background:

  • The toxicity of dental monomers like 2-hydroxyethylmethacrylate (HEMA) and triethleneglycoldimethacrylate (TEGDMA) is suspected to stem from oxidative metabolites.
  • Investigating protective mechanisms against these monomer toxicities is crucial for dental material safety.

Purpose of the Study:

  • To evaluate the protective effects of antioxidative vitamins and uric acid against HEMA and TEGDMA toxicity.
  • To compare the sensitivity of different toxicity markers, including glutathione depletion and protein synthesis inhibition.

Main Methods:

  • Assessed toxicity of HEMA and TEGDMA in L2, A549, and 11Lu cell lines.
  • Measured inhibition of methionine incorporation (protein synthesis) and glutathione (GSH) depletion.

Related Experiment Videos

  • Determined the impact of Vitamins C, E, A, and uric acid on HEMA/TEGDMA toxicity.

    • Main Results:

    • Glutathione depletion was the most sensitive indicator of HEMA and TEGDMA toxicity.
    • Vitamins C and E significantly mitigated HEMA/TEGDMA toxicity across tested cell lines.
    • Vitamin A and uric acid showed cell-specific protective effects against monomer toxicity.

    Conclusions:

    • The findings support the hypothesis that HEMA and TEGDMA toxicity involves radical metabolites.
    • Antioxidative substances effectively reduce monomer-induced toxicity, highlighting the role of the glutathione redox system.