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Related Concept Videos

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Related Experiment Video

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High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Ets ternary complex transcription factors.

Gilles Buchwalter1, Christian Gross, Bohdan Wasylyk

  • 1Institut de Génétique et Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries, BP 101142, CNRS, INSERM, ULP, 67404 Illkirch Cedex, France.

Gene
|December 25, 2003
PubMed
Summary
This summary is machine-generated.

Ternary complex factors (TCFs) like Elk-1, Net, and Sap-1 are crucial transcription factors. They regulate gene expression by forming complexes with SRF on the c-fos promoter, linking extracellular signals to gene activity.

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Transcription Factors

Background:

  • Ternary complex factors (TCFs), including Elk-1, Net, and Sap-1, are a subfamily of Ets domain transcription factors.
  • TCFs form a ternary nucleoprotein complex with serum response factor (SRF) on the serum response element (SRE) of the c-fos promoter.
  • TCFs are key regulators of gene expression in response to extracellular signaling via the mitogen-activated protein (MAP) kinase pathway.

Purpose of the Study:

  • To review molecular, structural, and biochemical studies on TCFs.
  • To elucidate the functional domains, complex formation, and transcriptional regulation by TCFs.
  • To discuss TCF protein partners, target genes, and emerging in vivo biological roles.

Main Methods:

  • Review of molecular studies.
  • Analysis of structural and biochemical data.
  • Summary of cell line and in vivo studies.

Main Results:

  • Detailed understanding of TCF functional domains and ternary complex formation with SRF.
  • TCFs act as effectors in signaling pathways and gene regulation.
  • Identification of TCF protein partners and target genes.

Conclusions:

  • TCFs are central to transcriptional regulation and gene expression dynamics.
  • Extensive research has clarified TCF mechanisms in vitro.
  • Emerging in vivo studies are expanding our knowledge of TCF functions.