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Updated: Aug 29, 2026

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
[The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane]
Changzhen Wang1, Jianbo Cong, Hong Xian
1Institute of Radiation Medicine Academy of Military Medical Science, Beijing 100850, China.
Objective:
To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane.
Method:
Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer.
Results:
The mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly.
Conclusion:
The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.
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