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[Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts]
Chonglin Song1, Zhifu Zhang, Xue Chen
1National Engine Combustion Laboratory, Tianjin University, Tianjin 300072, China.
Objective:
To study DNA damage of three kinds of gasoline oxygenates.
Method:
Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts.
Results:
In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative.
Conclusion:
Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.
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