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Related Experiment Videos

Sensitivity of OR in phage lambda.

Audun Bakk1, Ralf Metzler, Kim Sneppen

  • 1NORDITA (Nordic Institute for Theoretical Physics), DK-2100 Copenhagen, Denmark. audunba@nordita.dk

Biophysical Journal
|December 26, 2003
PubMed
Summary

Bacteriophage lambda

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Area of Science:

  • Molecular Biology
  • Genetics
  • Systems Biology

Background:

  • Bacteriophage lambda's regulatory network controls its life cycle.
  • The right operator is a key genetic element in this regulation.
  • Understanding its sensitivity to protein concentrations is crucial.

Purpose of the Study:

  • To investigate the sensitivity of bacteriophage lambda's right operator.
  • To analyze this sensitivity across different regulatory protein concentration regimes.
  • To identify suppressors that can restore wild-type activity after perturbations.

Main Methods:

  • Probing the system in three distinct regulatory protein concentration regimes.
  • Systematic perturbations of protein-operator binding energies.
  • Analyzing activity at promoters PRM and PR.

Main Results:

  • Promoter PRM activity is robust in the lysogen regime (CI dominates).
  • Promoter PR shows high sensitivity in the lysogen regime.
  • Both promoters exhibit high sensitivity during and after induction (CI and Cro comparable/Cro dominates).
  • Suppressors were identified in all regimes, compensating for binding energy perturbations.

Conclusions:

  • The sensitivity of bacteriophage lambda's right operator varies significantly with regulatory protein concentrations.
  • The system exhibits robustness in specific conditions but sensitivity in others.
  • Compensatory mechanisms (suppressors) exist to maintain wild-type activity despite perturbations.

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