Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Skin Cancer01:30

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adherence to the WCRF/AICR cancer prevention recommendations and mammographic density.

Breast cancer research : BCR·2026
Same author

CD44v6 is associated with tumor aggressiveness and chemoresistance in bladder cancer.

Scientific reports·2026
Same author

Integrating circulating microRNAs with epidemiological factors enhances breast cancer detection across subtypes: the MCC-Spain study.

Scientific reports·2026
Same author

Evolution of outdoor sports during the COVID-19 post-lockdown in Spain: a nationwide population-based study.

BMC public health·2026
Same author

Anthropometric Characteristics of Triple-Negative Breast Cancer Patients by Menopausal Status: Evidence from the Population-Based Multicentric Study-MCC-Spain.

Healthcare (Basel, Switzerland)·2026
Same author

Association between arsenic levels in toenails and urine and prostate cancer risk: Findings from the MCC-Spain study.

Environmental research·2026

Related Experiment Video

Updated: Jul 16, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Progression in cutaneous malignant melanoma is associated with distinct expression profiles: a tissue

Soledad R Alonso1, Pablo Ortiz, Marina Pollán

  • 1Molecular Pathology Program and Histology and Immunohistochemistry Unit, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid.

The American Journal of Pathology
|December 26, 2003
PubMed
Summary

This study identified biological markers that indicate melanoma progression and patient survival. Key cell cycle regulators like cyclin D1, p16(INK4a), and p27(KIP1) showed altered expression across melanoma stages, aiding in survival prediction.

More Related Videos

A 3D Organotypic Melanoma Spheroid Skin Model
08:49

A 3D Organotypic Melanoma Spheroid Skin Model

Published on: May 18, 2018

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Related Experiment Videos

Last Updated: Jul 16, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

A 3D Organotypic Melanoma Spheroid Skin Model
08:49

A 3D Organotypic Melanoma Spheroid Skin Model

Published on: May 18, 2018

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Area of Science:

  • Oncology
  • Dermatology
  • Molecular Biology

Background:

  • Cutaneous malignant melanoma is a significant cause of cancer death.
  • Clinical and histological factors predict melanoma survival, but biological markers require further investigation.
  • Understanding melanoma progression at a molecular level is crucial for improving patient outcomes.

Purpose of the Study:

  • To identify biological variables and expression profiles that distinguish melanoma progression stages (radial, vertical, metastases).
  • To explore the role of cell cycle regulators in melanoma development.
  • To develop a predictive model for overall survival in vertical growth phase melanoma patients.

Main Methods:

  • Retrospective analysis of 165 melanoma samples from 88 patients using tissue microarrays (TMAs).
  • Utilized a panel of 39 antibodies targeting proteins involved in cell cycle, apoptosis, melanoma antigens, and DNA repair.
  • Validated a predictive model using an independent cohort of 72 vertical growth phase melanoma patients.

Main Results:

  • Identified distinct protein expression profiles correlating with melanoma progression stages.
  • Observed significant alterations in cell cycle regulators: cyclin D1 decreased in vertical melanoma, while p16(INK4a) and p27(KIP1) diminished in advanced stages.
  • Developed and validated a four-antibody (Ki67, p16(INK4a), p21(CIP1), Bcl-6) predictor model for shorter overall survival in vertical growth phase melanoma.

Conclusions:

  • Specific biological markers and expression profiles can differentiate melanoma progression.
  • Cell cycle dysregulation, particularly affecting G1/S transition, is a key feature of melanoma advancement.
  • The validated four-marker model offers a promising tool for predicting survival in vertical growth phase melanoma, aiding clinical decision-making.