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Related Experiment Videos

Albumin enhanced morphometric image analysis in CLL.

Matthew A Lunning1, Vincent E Zenger, Ricardo Dreyfuss

  • 1Flow and Image Cytometry Section, Laboratory Stem Cell Biology, Division of Cell and Gene Therapies, Center for Biologics Research and Evaluation, Food and Drug Administration, Bethesda, Maryland 20892, USA.

Cytometry. Part B, Clinical Cytometry
|December 30, 2003
PubMed
Summary
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Adding bovine serum albumin (BSA) to blood films reduces atypical lymphocytes and smudge cells in chronic lymphocytic leukemia (CLL) samples. This method improves cell morphology analysis for better disease subclassification.

Area of Science:

  • Hematology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Chronic lymphocytic leukemia (CLL) presents lymphocyte heterogeneity.
  • Blood film artifacts complicate morphologic subclassification of CLL.

Purpose of the Study:

  • To evaluate the effect of bovine serum albumin (BSA) on blood film preparation for chronic lymphocytic leukemia (CLL) analysis.
  • To determine if BSA improves the accuracy of morphologic subclassification of CLL.

Main Methods:

  • Paired blood films from 82 CLL patients were prepared using EDTA with or without BSA.
  • Digital photomicroscopy and statistical analysis were performed on selected slides.
  • Lymphocyte and nuclear areas, nuclear transmittance, and nuclear:cytoplasmic ratios were measured.

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Main Results:

  • BSA addition significantly decreased mean cell area and nuclear area.
  • BSA significantly increased nuclear transmittance and nuclear:cytoplasmic ratio.
  • BSA addition reduced the number of atypical lymphocytes and smudge cells.

Conclusions:

  • Bovine serum albumin (BSA) addition to blood films aids in reducing atypical lymphocytes and smudge cells in CLL.
  • BSA decreases lymphocyte and nuclear size while increasing nuclear transmittance and nuclear:cytoplasmic ratio.
  • Standardized slide preparation with BSA may enhance morphologic subclassification of CLL, improving correlation with cytogenetics and immunophenotyping.