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Cutaneous leishmaniasis in Multan: species identification
S Ayub1, M Gramiccia, M Khalid
1Department of Dermatology, Nishtar Hospital Multan, Pakistan.
JPMA. the Journal of the Pakistan Medical Association
|December 31, 2003
Summary
This study identified Leishmania tropica as the cause of cutaneous leishmaniasis in Multan. Researchers isolated and identified the parasite from patient lesions using isoenzyme electrophoresis.
Area of Science:
- Medical Parasitology
- Infectious Diseases
- Dermatology
Background:
- Cutaneous leishmaniasis is a significant public health concern.
- Accurate identification of Leishmania species is crucial for effective treatment and control strategies.
- Previous data on Leishmania species prevalent in Multan, Pakistan, were limited.
Purpose of the Study:
- To determine the specific Leishmania species responsible for cutaneous leishmaniasis cases in Multan, Pakistan.
- To provide etiological data for public health interventions.
Main Methods:
- Parasites were isolated from skin lesions of 30 confirmed cutaneous leishmaniasis patients using fine needle aspiration (FNA).
- Isolated parasites were cultured in Evans Tobie's medium.
- Species identification was performed using isoenzyme electrophoresis.
Main Results:
- Leishmania isolates were successfully obtained from 16 patients.
- All identified Leishmania strains belonged to Leishmania tropica zimodeme MON7 variant PGD.
- This confirms Leishmania tropica as the etiological agent.
Conclusions:
- Leishmania tropica is the primary species causing cutaneous leishmaniasis in the studied population of Multan.
- The findings highlight the importance of accurate species identification for targeted leishmaniasis management.
- Further research on transmission dynamics and vector control is recommended.