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Updated: Aug 29, 2026
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
Published on: May 3, 2017
Continuous measurement of glutamate and hydrogen peroxide using a microfabricated biosensor for studying the
Ryoji Kurita1, Katsuyoshi Hayashi, Keiichi Torimitsu
1NTT Advanced Technology, 3-1 Morinosato-Wakamiva, Atsugi, Kanagawa 243-0124, Japan.
Abstract:
We first measured the effects of trace levels of an endocrine disruptor, tributyltin (TBT), on the secretion response from nerve cells using a microfabricated biosensor designed for the continuous measurement of L-glutamate and hydrogen peroxide. We observed higher and long-lasting glutamate and hydrogen peroxide concentrations from the cells when cultured rat cortical neurons were exposed to TBT. Glutamate and hydrogen peroxide release was induced even when we reduced the TBT concentration to 10 nM. This concentration is about two orders of magnitude lower than the concentration that induced apoptosis-like cell death. We also report on the effects of NMDA and non-NMDA receptor antagonists, which can help us to understand the mechanism of TBT neurotoxicity.
