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Determination of nitrite by flow injection spectrophotometry using a home-made flow cell detector
Shizuko Hirata1, Bhavaniamma Vijayalekshmi Amma, Sathrugnan Karthikeyan
1Marine Resources and Environment, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hiro-suehiro, Kuire 737-0197, Japan. s-hirata@aist.go.jp
Abstract:
A simple, rapid, low cost and accurate determination using flow injection spectrophotometry with a home made flow cell detector, in combination with green LED and photodiode, had been developed for nitrite based on the Griess-Saltzman reaction, measuring the absorbance at 525 nm. The proposed system is able to monitor nitrite in freshwater samples at a throughput of 60 samples per hour with a relative standard deviation (%RSD) of less than 2.4% and a detection limit of <7 microg dm(-3). The calibration graph was linear between 0.010 - 1.0 mg dm(-3).