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Updated: Aug 29, 2026
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
Published on: September 25, 2019
[The primary study on the anti-HBV effect of whole recombinant yeast]
Yan Zeng1, Da-chuan Cai, Ming-li Peng
1The Institute for Viral Hepatitis, Chongqing University of Medical Science, Chongqing 400010, China.
Objectives:
Based on the immunologic character of Pichia pastoris yeast, a new therapeutic vaccine, whole recombinant yeast, was used to explore a new way to activate cell-mediated anti-viral immunity.
Methods:
The recombinant plasmids, pPIC9K/S and PIC9K/hsp(1-370)-S, were constructed by inserting the gene encoding HBsAg, HSP70 (1-370) -HBsAg into vector pPIC9K and then the recombinants were transfected into Pichia pastoris yeast,GS115, respectively. Then that recombinant yeast immunized BALB/C mice were detected for humoral and cellular immunity to HBsAg.
Results:
Recombinant yeast successfully activated the humoral immunity to HBsAg in mice, but failed to activate the cellular immunity.
Conclusion:
The whole recombinant yeast can be used as vaccine, but need further study for optimal way of immunization.
