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Plastic substrates based separation channels in electromigration techniques.

Jana Charvátová1, Zdenek Deyl, Miroslav Klevar

  • 1Institute of Physiology, Academy of Science of the Czech Republic, Vídenská 1083, 14220 Prague 4, Czech Republic.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|December 31, 2003
PubMed
Summary

Polymethylmethacrylate (PMMA) chip separations using alpha-cyclodextrin achieved results comparable to fused silica capillaries. Plastic materials showed distinct electroosmotic flow in SDS-containing electrolytes due to sorption.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Plastic materials offer potential for cost-effective separation devices.
  • Challenges exist in achieving high selectivity and stable electroosmotic flow in plastic-based separation systems.

Purpose of the Study:

  • To evaluate polyester, polyurethane, and polymethylmethacrylate as materials for capillary electrophoresis (CE) and chip electrophoresis (CZE) separation media.
  • To assess the impact of alpha-cyclodextrin on separation selectivity in plastic channels.
  • To investigate electroosmotic flow (EOF) behavior in plastic separation channels.

Main Methods:

  • Testing polyester and polyurethane capillaries and polymethylmethacrylate (PMMA) chips for separation.
  • Utilizing a mixture of 11 fluorescein isothiocyanate amino acid derivatives as analytes.

Related Experiment Videos

  • Employing background electrolytes with and without alpha-cyclodextrin, including SDS-containing electrolytes.

    • Main Results:

    • Polymethylmethacrylate (PMMA) chips with 40 mmol/l alpha-cyclodextrin in the background electrolyte yielded separation selectivity comparable to bare fused silica capillaries.
    • All tested plastic materials exhibited significant electroosmotic flow (EOF) in sodium dodecyl sulfate (SDS) containing background electrolytes, attributed to sorption.
    • Histidine showed altered migration times in polystyrene and PMMA channels compared to silica and polyurethane.

    Conclusions:

    • Polymethylmethacrylate (PMMA) is a promising material for chip electrophoresis, especially when optimized with additives like alpha-cyclodextrin.
    • Sorption effects on plastic surfaces significantly influence electroosmotic flow (EOF) in the presence of charged surfactants.
    • Plastic-based separation systems require careful consideration of material-electrolyte interactions to achieve optimal performance.