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Updated: Aug 29, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Application of liquid chromatography-electrospray ionization mass spectrometry for study of steroid-converting
Ivan Miksík1, Katerina Mikulíková, Jirí Pácha
1Institute of Physiology, Academy of Sciences of the Czech Republic, Vídenská 1083, CZ 142 20 Prague 4, Czech Republic. miksik@biomed.cas.cz
Abstract:
A high-performance liquid chromatography-atmospheric pressure ionization-electrospray ionization mass spectrometry (HPLC-API-ESI-MS) method was developed for the analysis of steroids in a study of steroid-converting enzymes. Separations ware done on a Zorbax Eclipse XDB-C18 column (eluted with a linear methanol-water-acetic acid gradient) and identification of the steroids involved was done by API-ESI-MS using positive ion mode and extracted ion analysis. The applicability of the present method for studying steroid metabolism was proven in assaying two steroid-converting enzymes (20beta-hydroxysteroid dehydrogenase and 11beta-hydroxysteroid dehydrogenase) in various biological samples (rat and chicken intestine, chicken oviduct).
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