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In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos
Published on: August 3, 2012
IL-1 beta-mediated neuropeptide and immediate early gene mRNA induction is defective in Lewis hypothalamic cell
Rongtai Wei1, Esther M Sternberg
1Integrative Neural Immune Program, NIMH, NIH, 36 Convent Drive, Building 36, Room 1A23, Bethesda, MD 20892-4020, USA.
Abstract:
We previously found that Lewis (LEW/N) hypothalamic cells respond to interleukin-1beta (IL-1beta) with reduced corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) peptide synthesis and secretion compared to Fischer (F344/N) cells. To investigate whether this peptide hyporesponsiveness in LEW/N cells is secondary to their deficient mRNA expression, temporal mRNA expression patterns of CRH, AVP, and several hypothalamic neuropeptides induced by IL-1beta in LEW/N and F344/N hypothalamic dissociated cell cultures were delineated by quantitative real-time polymerase chain reaction (RT-PCR). To investigate the molecular mechanisms underlying neuropeptide mRNA induction in cells of both strains, temporal mRNA expression patterns of immediate early genes (IEGs) and several signal transduction-associated molecules were also examined. We found that LEW/N hypothalamic cells were hyporesponsive to IL-1beta induction of neuropeptide and IEG mRNA, while LEW/N cells transcribed more IL-1 receptor and inducible nitric oxide synthase (iNOS) compared to F344N/N cells, suggesting that LEW/N and F344/N hypothalamic cells are differentially activated by IL-1beta.
