Related Experiment Video
Updated: Aug 29, 2026

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Published on: May 26, 2023
In vivo observation of internal limiting membrane in an eye with macular hole
1Department of Ophthalmology, Osaka Rosai Hospital, Osaka, Japan. toyagi@orh.go.jp
Purpose:
To determine whether an internal limiting membrane (ILM) in the foveal area with a full-thickness idiopathic macular hole (MH) was intact.
Design:
Interventional case report.
Methods:
The ILM was stained with indocyanine green and peeled with forceps during pars plana vitrectomy for a full-thickness MH with a cuff. The stained membrane over the MH was carefully examined to determine whether a hole was present.
Results:
The ILM was clearly observed by indocyanine green staining during pars plana vitrectomy; no hole was detected in the membrane over the MH.
Conclusions:
A full-thickness MH with a cuff can occur in the absence of a hole in the ILM.

