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Related Experiment Videos

Intracellular Ca dynamics in ventricular fibrillation.

Chikaya Omichi1, Scott T Lamp, Shien-Fong Lin

  • 1Division of Cardiology, Cedars-Sinai Medical Center and Center for Health Sciences, University of California-Los Angeles Cardiovascular Research Laboratory, David Geffen School of Medicine, UCLA, Los Angeles, California 90095-1760, USA.

American Journal of Physiology. Heart and Circulatory Physiology
|January 6, 2004
PubMed
Summary

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During ventricular fibrillation (VF), intracellular calcium (Cai) dynamics become uncoupled from membrane voltage (Vm). This suggests non-voltage-gated calcium release may disrupt cardiac electrical activity and wave propagation.

Area of Science:

  • Cardiac Electrophysiology
  • Calcium Signaling
  • Arrhythmia Mechanisms

Background:

  • Membrane voltage (Vm) and intracellular calcium (Cai) are coupled in the heart, influencing action potential duration (APD).
  • At rapid heart rates, sarcoplasmic reticulum Cai cycling may exhibit independent dynamics.
  • Non-voltage-gated Cai release could alter local APD and refractoriness, potentially causing wavebreak during ventricular fibrillation (VF).

Purpose of the Study:

  • To investigate the association between intracellular calcium (Cai) and membrane voltage (Vm) during ventricular fibrillation (VF).
  • To determine if Cai dynamics are voltage-dependent during rapid cardiac rhythms.

Main Methods:

  • Optical mapping of Cai transients using rhod 2 AM in isolated swine right ventricles.

Related Experiment Videos

  • Simultaneous intracellular membrane potential recording via microelectrodes or voltage-sensitive dye RH-237.
  • Quantitative analysis using mutual information (MI) to assess Vm-Cai association and frequency analysis (fast Fourier transform).

    • Main Results:

    • Mutual information between Vm and Cai significantly decreased during VF compared to pacing and ventricular tachycardia (VT).
    • Spatial correlation between Cai waves and Vm depolarization waves was lost during VF.
    • Dominant frequencies of Vm and Cai signals differed significantly during VF, unlike during VT.

    Conclusions:

    • Intracellular calcium (Cai) is closely associated with membrane voltage (Vm) during normal pacing and VT, but not during VF.
    • Findings suggest the occurrence of non-voltage-gated Cai release events during VF.
    • These localized Cai releases may contribute to wavebreak by locally altering Vm and APD.