An alpha-tocopherol dose response study in Paramecium tetraurelia

Vitamin E (D,L-alpha-tocopherol) was administered to Paramecium tetraurelia in doses of 10, 100, 1000 and 10,000mg/l throughout its clonal lifespan. ANOVA revealed significant differences in clonal lifespan between groups, whether lifespan was measured in total fissions, or in days (P<0.05). When mean clonal lifespan was measured in fissions the greatest difference was between the 1000mg/l alpha-tocopherol treatment at 382 fissions, and the ethanol control at 255.5 fissions. The greatest difference in mean clonal lifespan in days survived was between the 10,000mg/l alpha-tocopherol treatment at 292.5 days and the ethanol control at 76 days. ANOVA also revealed significant differences (P<0.05) in the initial cell fission rates between groups. At the 1000 and 10,000mg/l concentrations of alpha-tocopherol, a decrease in cell fission rates was apparent early in the lifespan, but these rates began to increase gradually during the late clonal lifespan. Although no clonal toxicity effects were found in terms of decreasing life-expectancy, the 1000 and 10,000mg/l treatment groups exhibited higher background mortality rates throughout their respective lifespans than did the control groups, which could represent a cytotoxic effect.