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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Comparison of the collecting efficiency of microbiological air samplers
1National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, Japan. shintani@nihs.go.jp
Abstract:
It is important to evaluate the prevention of nosocomial infections caused by airborne micro-organisms in healthcare facilities. For that purpose, the use of an air sampler is required. A comparison of the collecting efficiency of air samplers has not been studied so far using a single culture medium. Use of the same culture medium is necessary because of the variation of the cultivation efficiency with different culture media. In this study a statistically significant difference in collecting efficiency among air samplers was observed in case of SCDA (soybean casein digest agar) culture medium, but not SCDALP (soybean casein digest agar lecithin polysorbate) culture medium. In addition, a statistically significant difference of the cultivation efficiency was observed between SCDA and SCDALP culture media (P<0.05). These results suggest that any air sampler can be used with SCDALP culture medium.

