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Updated: Aug 29, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
In vivo interaction between CDKA and eIF4A: a possible mechanism linking translation and cell proliferation
Andrew P Hutchins1, Gethin R Roberts, Clive W Lloyd
1Department of Cell and Developmental Biology, John Innes Centre, NR4 7UH, Norwich, UK.
Abstract:
In a proteomics-based screen for proteins interacting with cyclin-dependent protein kinase (CDK), we have identified a novel CDK complex containing the eukaryotic translation initiation factor, eIF4A. Reciprocal immunoprecipitations using antibodies against eIF4A indicate that the interaction is specific. The CDKA-eIF4A complex is abundant in actively proliferating and growing cells but is absent from cells that have ceased dividing. The CDKA-eIF4A complex contains kinase activity that is sensitive to the CDK-specific inhibitor roscovitine. This interaction points to a possible molecular mechanism linking cell proliferation with translational control.
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