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Updated: Aug 29, 2026

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
Published on: July 20, 2019
Fibronectin is a hypoxia-independent target of the tumor suppressor VHL
Hans A R Bluyssen1, Martijn P J K Lolkema, Moniek van Beest
1Department of Medical Oncology, University Medical Center Utrecht, P.O. Box 85500, 3500 GA, Utrecht, The Netherlands.
Abstract:
The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.
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