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Related Experiment Videos

Cell shape and plasma membrane alterations after static magnetic fields exposure.

A Chionna1, M Dwikat, E Panzarini

  • 1Dept. of Biological and Environmental Science and Technology, University of Lecce, Italy.

European Journal of Histochemistry : EJH
|January 7, 2004
PubMed
Summary

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Static magnetic fields (SMFs) impact cell structure and calcium levels in lymphocytes and U937 cells. These SMF-induced changes, including altered cell shape and membrane microvilli, may influence cell survival pathways.

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Static magnetic fields (SMFs) are increasingly studied for their biological effects.
  • Understanding cellular responses to SMFs is crucial for potential therapeutic applications.
  • Apoptosis, or programmed cell death, is a fundamental biological process influenced by various environmental factors.

Purpose of the Study:

  • To investigate the biological effects of 6 mT static magnetic fields on human lymphocytes and U937 cells.
  • To analyze structural modifications in cell shape and plasma membrane.
  • To determine the role of intracellular calcium ([Ca++] ) in mediating these effects.

Main Methods:

  • Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed.

Related Experiment Videos

  • Lectin cytochemistry with ConA-FITC conjugates assessed plasma membrane changes.
  • Cells were exposed to static MFs with and without apoptosis-inducing drugs.

    • Main Results:

    • Static MFs altered cell shape, plasma membrane structure, and increased intracellular calcium levels.
    • Observed changes included the formation of lamellar microvilli and altered cell surface smoothness.
    • These modifications occurred independently of cell type or the presence of apoptotic drugs and were time-dependent.

    Conclusions:

    • Static magnetic fields exert discernible biological effects on human lymphocytes and U937 cells.
    • SMF exposure influences cell morphology and potentially anti-apoptotic pathways via calcium modulation.
    • Further research is needed to elucidate the role of these modifications in disease pathogenesis.