Related Experiment Video

Updated: Aug 29, 2026

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
06:04

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Published on: February 24, 2023

[Development of a competitive immunoassay based on monoclonal antibodies for the detection of specific antibodies to

V A Fedorova, Zh G Sameliia, Z L Devdariani

    Klinicheskaia Laboratornaia Diagnostika
    |January 8, 2004
    PubMed

    More Related Videos

    Generation of Monoclonal Antibodies Against Natural Products12:15

    Generation of Monoclonal Antibodies Against Natural Products

    Published on: April 6, 2019

    Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens09:08

    Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

    Published on: September 12, 2016

    Related Concept Videos

    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.