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Published on: April 11, 2016
[A simple method to detect disaccharides deficiency]
M Laguens1, J Collura, R Marrone
1Centro Gastroenterológico Platense, Calle 3 No 1095 (1900), La Plata, Argentina.
Abstract:
A simple method, easy to perform during an endoscopic procedure, fast and inexpensive, that allows detecting deficiencies in lactase, sucrase or maltase activities is presented. Briefly, method consists in placing a duodenal biopsy sample in an adequate vial containing lactose, sucrose or maltose solution during a few minutes, and then, adding a few drops of a glucose reactive from commercial origin. Presence of any enzymatic activity is demonstrated when released glucose from any of the disaccharides chosen reacts with the second reactive, turning solution to a red colour. Its utility is discussed and compared with other diagnostic methods.
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