Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A simple method to detect disaccharides deficiency].

M Laguens1, J Collura, R Marrone

  • 1Centro Gastroenterológico Platense, Calle 3 No 1095 (1900), La Plata, Argentina.

Acta Gastroenterologica Latinoamericana
|January 8, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pragmatic clustered randomised control trial to evaluate a self-regulated learning intervention to be implemented in South Australian primary schools-study protocol.

Trials·2025
Same author

Screening for Neglected Tropical Diseases and other infections in African refugees and asylum seekers in Rome and Lazio region, Italy.

Travel medicine and infectious disease·2023
Same author

What hides beneath the scar: sexuality and breast cancer what women don't say: A single-center study.

La Clinica terapeutica·2022
Same author

Effect of electrical water bath stunning on physical reflexes of broilers: evaluation of stunning efficacy under field conditions.

Poultry science·2016
Same author

Levels of Cadmium in White and Brown Meat of Warty Crab (Eriphia verrucosa).

Journal of food protection·2015
Same author

Salvage treatment with ganciclovir in a splenectomized, polytransfused patient affected by systemic inflammatory response syndrome.

International journal of immunopathology and pharmacology·2014

This study presents a fast, inexpensive endoscopic method to detect deficiencies in lactase, sucrase, or maltase enzymes by analyzing glucose release from disaccharides in duodenal biopsies.

Area of Science:

  • Gastroenterology
  • Biochemistry
  • Diagnostic Medicine

Background:

  • Disaccharide malabsorption, including lactase, sucrase, and maltase deficiencies, affects nutrient absorption and can cause gastrointestinal symptoms.
  • Accurate and timely diagnosis of these enzyme deficiencies is crucial for effective patient management.
  • Existing diagnostic methods may be time-consuming, expensive, or require specialized equipment.

Purpose of the Study:

  • To introduce a simple, rapid, and cost-effective method for detecting lactase, sucrase, and maltase deficiencies.
  • To enable in-situ enzymatic activity assessment during endoscopic procedures.
  • To compare the utility of this novel method with established diagnostic approaches.

Main Methods:

  • A duodenal biopsy sample is incubated with lactose, sucrose, or maltose solution.

Related Experiment Videos

  • A commercial glucose-reactive agent is added to the sample.
  • Enzymatic activity is indicated by a colorimetric change (red color) due to glucose release.

    • Main Results:

    • The method successfully detects the presence or absence of lactase, sucrase, and maltase activities.
    • The procedure is straightforward to perform during endoscopy.
    • The assay is rapid and utilizes inexpensive, commercially available reagents.

    Conclusions:

    • This novel method offers a practical and efficient approach for diagnosing disaccharidase deficiencies.
    • Its simplicity and speed make it suitable for immediate assessment during endoscopic examinations.
    • The technique holds potential as a valuable tool in routine gastroenterological diagnostics.