The aim of this article is to present a method for identifying and protecting the recurrent laryngeal nerve during robotic thyroidectomy through the oral vestibular approach in the absence of the laryngeal nerve...

Begin with an anesthetized human patient diagnosed with recurrent glioblastoma, an invasive tumor characterized by tumor cell infiltration into the surrounding peritumoral region.The peritumoral region retains an intact blood-brain barrier (BBB), where tight junctions between endothelial cells restrict drug penetration.Make an incision in the scalp, create an opening in the skull, and insert a laser probe into the tumor core using MRI guidance.Deliver laser light through the probe.Tissue...

This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin.  The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and fluorescence in situ hybridization...

The use of an RNA-based approach to determine quantitative immune profiles of solid tumor tissues and leverage clinical cohorts for immune-oncology biomarker discovery is described through a molecular and informatics...

We present protocols for the isolation of stable heterocyclic carbenes. The synthesis of a cyclic (alkyl)(amino) carbene (CAAC) and an N-heterocyclic carbene (NHC) is demonstrated using filter cannulas and Schlenk technique. We furthermore present the synthesis of the related oxygen-sensitive, electron-rich mixed “Wanzlick dimer” and the reduced stable organic...

Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...