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Updated: Aug 29, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
Application of molecular beam deflection time-of-flight mass spectrometry to peptide analysis
Rodolphe Antoine1, Isabelle Compagnon, Driss Rayane
1Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579, Université Lyon I et CNRS, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. antoine@lasim.univ-lyon1.fr
Abstract:
The application of molecular beam deflection time-of-flight mass spectrometry (MBD-TOFMS) to peptide identification is described. The technique permits a simultaneous measurement of molecular mass and electric dipole susceptibility. The mass and susceptibility are not strongly correlated, and the results can be presented as a two-dimensional map. The susceptibility provides a useful way to disperse isobaric and isomeric peptides, and at least for small peptides, the susceptibility is significantly different for different amino acid sequences. Results for peptides in the mass range 1000-2300 Da show that the mass and susceptibility lead to a higher identification score than mass spectra alone.
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