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Detection of Salmonella enterica serovar typhimurium by using a rapid, array-based immunosensor
Chris Rowe Taitt1, Yura S Shubin, Roselina Angel
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C. 20375, USA. crtaitt@cbmse.nrl.navy.mil
Applied and Environmental Microbiology
|January 9, 2004
Summary
A multianalyte array biosensor (MAAB) rapidly detects Salmonella Typhimurium in food and poultry. This biosensor shows promise for screening asymptomatic Salmonella infections in poultry farms.
Area of Science:
- Food safety
- Veterinary diagnostics
- Biosensor technology
Background:
- The multianalyte array biosensor (MAAB) offers simultaneous detection of multiple analytes.
- Rapid immunoassays are crucial for timely foodborne pathogen detection.
Purpose of the Study:
- To develop and validate rapid immunoassays using MAAB for Salmonella Typhimurium detection.
- To assess MAAB's efficacy in detecting Salmonella in various food matrices and poultry samples.
Main Methods:
- Development of 15-min and 1-h single-analyte sandwich immunoassays for Salmonella Typhimurium.
- Testing MAAB on spiked food samples (sausage, cantaloupe, egg, sprouts, chicken rinse).
- Evaluation of MAAB for detecting Salmonella in chicken excreta from different farm types.
Main Results:
- Salmonella Typhimurium was detected in spiked foodstuffs with minimal sample preparation.
- Assay sensitivity improved 10-fold by extending the protocol to 1 hour (limit of detection: 8 x 10^3 CFU/g in poultry excreta).
- MAAB detected Salmonella in private farm poultry excreta, indicating potential for screening.
Conclusions:
- MAAB is a viable tool for rapid Salmonella Typhimurium detection in diverse food products.
- MAAB demonstrates potential as a screening tool for asymptomatic Salmonella infections in poultry.
- Further validation is recommended for widespread application in poultry health monitoring.