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Related Experiment Video

Updated: Jul 22, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
12:11

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Published on: August 27, 2015

Protection from oxidative damage in mouse liver cells.

A W Harman1, G M Adamson, S G Shaw

  • 1Department of Pharmacology, University of Western Australia, Nedlands.

Toxicology Letters
|December 1, 1992
PubMed
Summary

Paracetamol toxicity in mouse liver cells involves oxidative stress from NAPQI. The drug Ebselen, acting as a glutathione peroxidase mimic, effectively reduced this oxidative damage.

Area of Science:

  • Hepatotoxicity
  • Oxidative Stress
  • Drug Metabolism

Background:

  • Paracetamol overdose is a leading cause of acute liver failure.
  • Hepatotoxicity is linked to the reactive metabolite N-acetyl-p-benzoquinone imine (NAPQI).
  • Oxidative stress plays a critical role in paracetamol-induced liver injury.

Purpose of the Study:

  • To investigate the role of oxidative stress in paracetamol toxicity in mouse hepatocytes.
  • To evaluate the efficacy of Ebselen in mitigating paracetamol-induced oxidative damage.

Main Methods:

  • Primary mouse hepatocytes were exposed to paracetamol.
  • Assessment of oxidative stress markers and cell viability.
  • Treatment with Ebselen to evaluate its protective effects.

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Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
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Last Updated: Jul 22, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
12:11

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Published on: August 27, 2015

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
12:59

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Published on: February 15, 2020

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
11:03

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Published on: September 9, 2021

Main Results:

  • Paracetamol exposure led to significant oxidative stress, primarily in later stages.
  • NAPQI formation was identified as a key initiator of oxidative events.
  • Ebselen treatment significantly ameliorated oxidative stress and protected hepatocytes.

Conclusions:

  • Oxidative stress is a major contributor to paracetamol hepatotoxicity.
  • Ebselen demonstrates potential as a therapeutic agent for paracetamol poisoning by reducing oxidative damage.