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Single-molecule DNA nanomanipulation: detection of promoter-unwinding events by RNA polymerase

A Revyakin1, J F Allemand, V Croquette

  • 1Howard Hughes Medical Institute, Waksman Institute, Department of Chemistry, Rutgers University, Piscataway, New York 08854, USA.

Methods in Enzymology
|January 10, 2004
PubMed
Abstract

No abstract available in PubMed .

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Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are: