Related Experiment Video

Updated: Aug 29, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
12:43

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Published on: November 12, 2017

Evaluation of fluorescence spectroscopy methods for mapping melted regions of DNA along the transcription pathway

Craig T Martin1, Andrea Ujvári, Chihua Liu

  • 1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003-9336, USA.

Methods in Enzymology
|January 10, 2004
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast09:19

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast

Published on: March 6, 2026

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...