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Formate buffer as preservative for urinary free catecholamine measurement.

Y P Iu1, C S Ho, T W L Mak

  • 1Department of Clinical Pathology, North District Hospital, Hong Kong Special Administrative Region, China.

Annals of Clinical Biochemistry
|January 10, 2004
PubMed
Summary

Formate buffer is an effective and safer alternative to strong mineral acid for preserving urinary free catecholamines. Deep-freeze storage further enhances preservation, reducing safety hazards associated with corrosive acids.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Urinary free catecholamines are commonly measured using strong mineral acid preservatives.
  • Corrosive nature of strong mineral acids poses safety hazards due to potential leakage.
  • Investigated formate buffer as a safer alternative preservative.

Purpose of the Study:

  • To evaluate the efficacy of formate buffer in preserving urinary free catecholamines.
  • To compare the preservation capability of formate buffer with hydrochloric acid.
  • To assess the impact of storage temperature on catecholamine stability.

Main Methods:

  • Collected 22 post-operative patient urine specimens.
  • Preserved specimens in 0.5 M hydrochloric acid or 0.75 M formate buffer.

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  • Stored samples at 4°C or -70°C and analyzed free catecholamines via HPLC for 6 months.

    • Main Results:

    • Formate buffer demonstrated preservation capabilities equivalent to hydrochloric acid.
    • Deep-freeze storage (-70°C) provided enhanced preservation, irrespective of the buffer used.
    • No significant degradation of urinary free catecholamines was observed with formate buffer over 6 months.

    Conclusions:

    • Formate buffer is an equally effective preservative for urinary free catecholamines compared to strong mineral acid.
    • The less acidic nature of formate buffer reduces safety risks and handling incidents.
    • Formate buffer offers a safer and viable alternative for routine urinary free catecholamine analysis.