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Related Experiment Videos

A first generation bovine BAC-based physical map.

Laurent Schibler1, Anne Roig, Marie-Françoise Mahé

  • 1Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas, France. schibler@jouy.inra.fr

Genetics, Selection, Evolution : GSE
|January 10, 2004
PubMed
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Researchers created a bovine genome physical map using clone fingerprinting and marker screening. This map aids genomic research in ruminants for targeted sequencing and gene discovery.

Area of Science:

  • Genomics
  • Comparative Genomics
  • Bioinformatics

Background:

  • Physical maps are essential for understanding genome organization and function.
  • Previous bovine genome mapping efforts have limitations in resolution and marker density.

Purpose of the Study:

  • To construct a first-generation, clone-based physical map of the bovine genome.
  • To provide a foundational resource for advanced genomic studies in cattle and other ruminants.

Main Methods:

  • Utilized fluorescent double digestion fingerprinting of Bacterial Artificial Chromosome (BAC) clones.
  • Integrated Sequence Tagged Site (STS) marker screening, including microsatellites, genes, ESTs, and BAC ends.
  • Assembled contigs from two large BAC libraries (Inra and CHORI-240).

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Main Results:

  • Successfully constructed a physical map comprising 6615 contigs from 100,923 BAC clones.
  • Anchored contigs using 1303 diverse markers.
  • The map represents a significant advancement in bovine genome physical mapping.

Conclusions:

  • The developed bovine physical map is a valuable tool for ruminant genomic research.
  • Facilitates targeted marker production, positional cloning, and sequencing of specific genomic regions.